Journal Club-Genome editing with RNA guided Cas9 nuclease in Zebrafish embryos

This is an original article published in early 2013 in Cell Research magazine. It mainly includes four parts of experiments: Cas9/gRNA ribonucleoprotein complex executes site-specific DNA cleavage in mammalian cells; Cas9/gRNA induces indels in the etsrp locus in zebrafish; Cas9/gRNA induces indels in...

Increased genome instability in human DNA segments with self-chains: homology-induced structural variations via replicative mechanisms

Hum. Mol. Genet. (2013) 22 (13): 2642-2651. Environmental factors including ionizing radiation and chemical agents have been known to be able to induce DNA rearrangements and cause genomic structural variations (SVs); however, the roles of intrinsic characteristics of the human genome, such as regional...

Replicative mechanisms in PTLS syndrome

Human Molecular Genetics, 2013, Vol. 22, No. 4 749–756 Copy number variations (CNVs) in the human genome contribute significantly to disease. De novo CNV mutations arise via genomic rearrangements, which can occur in ‘trans’, i.e. via interchromosomal events, or in ‘cis’, i.e. via...